Fig. 4

Representative composite of 6 month-old non-end-stage Tg53 proximal (≈400 micron) coronary lesion. (A) H&E staining reveals foam cell (fc)-rich lesion; intact internal elastic lamina (➩); leukocyte adhesion (➨); intact endothelium (▽); red blood cells (rbcs) in the vessel lumen; media (m); bar = 10 microns. (B) ED1 immunostained (➨) lesion foam cells and adhered leukocytes indicating monocyte-macrophage lineage; intact endothelium (▽); internal elastic lamina (➩); bar=10 microns. (C) Intense α-actin immunostained (red) smooth muscle cells in the lesion-side media; no immunostaining in intimal lesion; leukocyte adhesion (➨) onto the intact endothelium (▽); bar = 10 microns. (D) H&E stained distal coronary (≈160 microns) plaque with thick cap, delipidated extracellular lipid, intact endothelium (▼) and internal elastic lamina (➩). No thrombi, hemorrhage or neovascularization are detected. Identical H&E stained section to (A); bar = 25 microns. Note: (ad), adventitia; (fc), foam cells; (m), media; (rbcs), red blood cells.