Table 1 Primers and condition—PCR analysis of flanking regions of the STS gene
From: Deletion Pattern of the STS Gene in X-linked Ichthyosis in a Mexican Population
Locus | Primer Sequence | Annealing (°C) | (bp) |
|---|---|---|---|
DXS89 | F CGT TCT CAA CAA TAG ATC ACG R ACT TAC ATT GGC AAT GCA TCC | 50 | 166 |
DXS996 | F AAA TTC TTG CCT AGG CCA CTC TAG G R ACG TTG TTC TGG ATC GTA TGC TAG G | 62 | 153–171 |
DXS1139 | F GCT TGG ATC AGG GCT GGA ATT AG R TTA AAC AAG GGC CAT TCT GCA AG | 60 | 500 |
DXS1130 | F TGT CTC AAC CGT CAA GTC AC R CTT GTC TTT GAA GAT GCT CCC | 60 | 367 |
5′ GEN STS | F GGC CTA GAA GAA GGT TGA AGG TCC C R AAG AGG TTG GAT GAG ATG GGC ATA C | 60 | 292 |
3′ GEN STS | F GAA ATC CTC AAA GTC ATG CAG GAA G R CCT CCA GTT GAG TAG CTG TTG AGC T | 60 | 363 |
DXS1131 + | F CCA AAC TGC CAC TGG TTG AG R CCG TGA TCT CTG ATG AAC CG | 58 | 170 |
DXS1133 | F TGG TAG AAT ACA CTG CAG GTG TGC R CAG CTT GTT AAG TGC TGT CTA TCC | 60 | 260 |
DXS237 | F CAT GTG AGG AAT ACA GGA GCG AA R GCC AAC TCA GCT AAT GAC TGT AG | 55 | 400 |
DXS1132 | F TGC TAC AAG CAT TTC AGA GG R CAA GCT CTG ACA TGT GAC TTC | 55 | 152 |
DXF22S1 | F ATC CAC TCC CCA TCT TAC CTT TCC C R CAA ATA GAC CCC TTC TTG TCT GGG | 62 | 262 |
DXS278 | F CCT TGA GTC TTT GTT CTG GAA CCC R CTA CGT GGT TGA ATG CCT GGA CTC | 55 | 210 |
DXS1134 | F TGA GTA TGT GCT TTG AGC TAG GGT C R CTG CGA ACA ATT CTT TTA GTT CCT C | 50 | 400 |