Fig. 1

NS-398 effects on proliferation of prostate epithelial cells in culture. LNCaP or LNCaP C4-2b cells (1 × 10⁶) were plated in 6 well cluster plates for 24 h in standard medium, then medium was replaced with serum-free medium supplemented with 0.5 µg/ml BSA containing 10 µM NS-398. Cells were cultured for an additional 72 h with samples taken at 24 h intervals. Cell number was determined by direct counting using trypan blue dye exclusion. Data represents mean of three separate experiments performed in duplicate. Closed triangle, C4-2b control; open triangle, C4-2b, NS-398; closed circle, LNCaP control; open circle, LNCaP, NS-398. DMSO (0.1%) was added to control cultures. Error bars = SE of means.