Fig. 2
From: Cellular Penetration of Fluorescently Labeled Superoxide Dismutases of Various Origins
Results from incubation with FITC-SOD (1500 U/ml) for 3 hr as shown by confocal laser microscopy. (1) Recombinant human CuZn SOD, BTG; (2) CuZn SOD from bovine erythrocytes, Sigma; (3) CuZn SOD from bovine erythrocytes, Palleau Production; (4) E. coli Mn SOD, Sigma; (5) recombinant human Mn SOD, BTG; (6) CuZn SOD from equine erythrocytes, Palleau Production. The four CuZn SODs have the same intracellular diffuse distribution pattern, whereas the Mn SODs have poor labeling after 3 hr. (7) Two focal sections taken at a distance of 1 µm in the middle of the same cell. There is no nuclear labeling. (8) One of the rare FITC-labeled PMN. The single intracellular focal spot shows a distribution pattern in the cytoplasm that is different from that of monocytes.