Cellular Penetration of Fluorescently Labeled Superoxide Dismutases of Various Origins

Table 2 PMA-stimulated superoxide production by monocytes: Inhibitory effect of pretreatment with various SODs

Experiment	CuZn rh BTG	CuZn bovine Sigma	CuZn bovine Palleau	CuZn horse Palleau	Mn rh BTG	Mn E. coli Sigma
I	−62.2	−62.2	−75.6	−45.6 (−46.8)	+ 18.6 (−4.1)	−57.1
II	−39.9	−28.8	−34.2	−51.4 (−42.9)	+20.0 (+20.0)	−15.6
III	−36.1	−30.7	−44.4	−71.0 (−64.3)	−19.0 (−15.9)	−18.7
IV	−42.1	−46.8	−34.7	−70.7 (−73.1)	−29.9 (−29.6)	−20.5
Mean	−45.0*	−42.1*	−48.7*	−59.7 (−56.8)*	−2.6 (−7.4)^a	−28.0^a

Pretreatment of cells for 2 hr with 300 units/ml SOD followed by three washes; PMA concentration, 30 ng/ml. The results are expressed as percent inhibition of cytochrome c reduction over a 1-hr period. Results in brackets are from parallel experiments with FITC-labeled SOD.
^aNot significant.
*p < 0.02.

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