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Fig. 1 | Molecular Medicine

Fig. 1

From: Re-expression of SPR1 in Breast Cancer Cells by Phorbol 12-Myristate 13-Acetate (PMA) or UV Irradiation Is Mediated by the AP-1 Binding Site in the SPR1 Promoter

Fig. 1

Identification of the SPR1 gene by differential display on the basis of its down-regulated or absent expression in metastatic tumor cells. (A) Total RNAs from normal human mammary epithelial cell strains (76N, 70N) (lanes 1,2), primary (21NT, 21PT) (lanes 3,4), and metastatic breast tumor cell lines (21MT-2, 21MT-1) (lanes 5,6) were compared. The resultant cDNAs were resolved by electrophoresis on a 6% acrylamide/urea sequencing gel. The position of the SPR1 cDNA (∼0.16 kb) is marked. (B) Confirmation of SPR1 differential expression pattern by Northern blotting. Total RNAs from normal mammary epithelial cell strains (76N, 70N) (lanes 1,2), primary (21NT, 21PT) (lanes 3,4), and metastatic mammary epithelial tumor cell lines (21MT-2, 21MT-1) (lanes 5,6) were compared. Each lane contained 10 µg of total cellular RNA. Staining with ethidium bromide showed similar loading of non-degraded RNA in each lane (not shown). The size of the transcript was estimated from molecular size markers run on a parallel lane (not shown).

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Molecular Medicine

ISSN: 1528-3658