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Fig. 6 | Molecular Medicine

Fig. 6

From: Re-expression of SPR1 in Breast Cancer Cells by Phorbol 12-Myristate 13-Acetate (PMA) or UV Irradiation Is Mediated by the AP-1 Binding Site in the SPR1 Promoter

Fig. 6

An intact AP-1 site is required for enhancement of SPR1 promoter activity. CAT assays were performed in 70N normal breast cells (A) and MDA-MB-436 breast tumor cells (B) and the percentage of conversion of [14C] chloramphenicol to the acetylated form was measured. Values obtained from counting spots in a scintillation counter are shown (C). CAT constructs were transfected containing wild-type AP-1 (lane 4), and mutant AP-1 with two nucleotide changes (lane 5), or with changes in all seven nucleotides of AP-1 (lane 6). The pBLCAT3 promoterless vector and the pBLCAT2 vector with the TK promoter were transfected as negative controls (lanes 1–3). Extracts of 15 β-Gal units were assayed for CAT activity. Results are typical of triplicate experiments.

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Molecular Medicine

ISSN: 1528-3658