Fig. 3
Effects of leukotriene E4 on number of histamine H1 receptors in murine macrophages. The Scatchard plot represents the binding of [3H]-pyrilamine to murine macrophages, left unstimulated (open circles) or stimulated (filled circles) with LTE4 (100 ng/ml) for 10 min. Scatchard analysis was performed to determine number of receptors per cell as described in Materials and Methods. We measured 1.9 × 105 receptors/cell before stimulation and 4 × 105 receptors/cell after stimulation with a Kd value of 5.5 nM.