Volunteer
|
Receptors/cell before stimulation
|
Receptors/cell after stimulation
|
K
d
(nM)
|
---|
A
|
54207
|
105403
|
6
|
B
|
45173
|
90345
|
6
|
C
|
22586
|
48184
|
5.5
|
D
|
12046
|
57219
|
6.7
|
E
|
2259
|
9787
|
3
|
- Blood was isolated from five healthy volunteers (A–E) and monocytes were prepared. These cells were stimulated with leukotriene E4 (100 ng/ml) for 15 min. Scatchard analysis was performed to determine number of receptors per cell as described in Materials and Methods.