Influenza Virus Infection is not Affected by Serum Amyloid P Component

Table 2 Antibody response and viral titers during influenza virus infection in C57BL/6 wild type and SAP knockout mice

	Serum Antibody [Mean (SD)]		BAL Antibody [Mean (SD)]		Lung Viral Titer [TCID₅₀, Individual Values]
Day	Wild Type	SAP ko	Wild Type	SAP ko	Wild Type	SAP ko
3	59 (8)	94 (30)	25 (7)	17 (8)	3.75, 4.75, 4.75, NA	<0.75, <0.75, 2.5, 3.5
5	485 (194)	311(138)	13 (4)	17 (9)	3.75, 3.75, 4.5, 4.5	3.5, 4.5, 4.75, 5.0
8	4016 (777)	4350 (254)	300 (155)	58 (21)	1.25, 1.25, 1.5, 4.0	1.0, 1.5, 1.75, 2.75

Mice were infected intra-nasally on day 0 with the A/PR/8/34 strain of influenza virus, and batches of 4 animals were killed on days 3,5 and 8 to provide serum and bronchoalveolar fluid for anti-influenza virus antibody titration, and whole lung homogenates for virus titration. Total mouse anti-influenza virus antibody was estimated by solid phase immunoradiometric assay calibrated with a single standard high titer mouse antiserum to which a value of 1000 antibody units was assigned. There were no spontaneous deaths and the weights of all surviving animals on each day are shown in Fig. 2.

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