Fig. 1.
From: The Chediak-Higashi Protein Interacts with SNARE Complex and Signal Transduction Proteins
Biochemical confirmation of interactions of LYST with 14-3-3τ, CK2β, and HRS by an in vitro binding assay. Following binding, the eluted proteins were resolved by SDS-PAGE, Western blotted, and detected with specific antibodies. LYST N-terminal domain: nucleotides 190–1056. LYST WD-40 (C-terminal) domain: nucleotides 10576–11611. 14-3-3τ negative control: LYST C-terminus. CK2-β negative control LYST 6586–7449. HRS negative control: LYST N-terminus. All interactions were confirmed except that between CK2β and the LYST WD-40 domain. Molecular size standards (in kD) are shown. LYST nucleotide positions are derived from GenBank U67615.