Fig. 7
Effect of cholesterol depletion on the involvement of CIR in activation of pp59Lyn by PIG41. Isolated rat adipocytes were incubated in the absence (Control) or presence of 10 µg/ml trypsin plus 0.5 M NaCl. Portion of the trypsin/NaCl-treated adipocytes was reconstituted with trypsin/NaCl-extract. After further incubation (20 min, 37°C) in the absence or presence of 3 µM PIG41, lcDIGs were prepared and used for immunoprecipitation (IP) of pp59Lyn in dissociating buffer. The immunoprecipitates were immunoblotted (IB) for phosphotyrosine (pY). The figure shows chemiluminescent images of a typical experiment repeated three times with similar results. Quantitative evaluations of four different adipocyte incubations are given as means (arbitrary units) with PIG41-treated control cells set at 100.