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Table 4 Effect of cholesterol depletion on stimulation of glycogenesis and glycogen synthase

From: Cholesterol Depletion Blocks Redistribution of Lipid Raft Components and Insulin-Mimetic Signaling by Glimepiride and Phosphoinositolglycans in Rat Adipocytes

 

Glycogenesis

Glycogen Synthase

 

Control

mβCD

mβCD + mβCD-chol

Control

mβCD

mβCD + mβCD-chol

Basal

1.0 ± 0.2

1.3 ± 0.3

1.2 ± 0.2

1.0 ± 0.3

1.2 ± 0.2

0.9 ± 0.2

Na3VO4

3.9 ± 0.5

3.3 ± 0.4

4.0 ± 0.6

2.6 ± 0.4

2.9 ± 0.5

3.1 ± 0.4

POV

4.9 ± 0.9

5.2 ± 0.8

5.4 ± 0.7

3.0 ± 0.6

3.3 ± 0.4

3.5 ± 0.5

Insulin

6.8 ± 1.2

2.5 ± 0.4

5.9 ± 0.9

3.5 ± 0.7

1.5 ± 0.3

3.2 ± 0.3

PIG41

7.5 ± 1.8

2.9 ± 0.5

6.3 ± 0.8

3.9 ± 0.8

1.3 ± 0.3

3.4 ± 0.4

Glim

2.7 ± 0.6

1.2 ± 0.3

3.0 ± 0.4

2.4 ± 0.4

1.1 ± 0.2

2.7 ± 0.3

  1. Isolated rat diaphragms were incubated (50 min,30°C) in the absence (control) or presence of 2.3 mM mβCD or 1.1 mM mβCD plus 1.1 mM mβCD-cholesterol complex (mβCD + mβCD-chol) and subsequently with 1 mM Na3VO4, 10µM potassium bisperoxo [1,10-phenanthroline] oxovanadate (POV), 50 nM insulin, 3 µM PIG41, or 10 µM glimepiride (50 min, 37°C). The diaphragms were assayed for glycogenesis or glycogen synthase fractional velocity. Fold stimulations are given with basal values (absence of insulin) obtained with control cells set at 1. Each value represents the mean ± SD of three independent diaphragms with single incubations each.
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Molecular Medicine

ISSN: 1528-3658