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Fig. 1 | Molecular Medicine

Fig. 1

From: Anti-Tumor Efficacy of a Novel Antisense Anti-MDM2 Mixed-Backbone Oligonucleotide in Human Colon Cancer Models: p53-Dependent and p53-Independent Mechanisms

Fig. 1

Effects of anti-MDM2 antisense oligonucleotides on MDM2, p53, and p21 protein levels in LS174T (A) and DLD-1 (B) cells in culture. Cells were incubated with Oligo AS at various concentrations for 24 hr, in the presence of lipofectin (7 µg/ml). Identical total protein (20 µg) was analyzed by SDS-PAGE, followed by Western blotting. Inhibitory effects of Oligo AS on MDM2 expression are shown in a dose-dependent manner, with greater than 90% inhibition be observed at 500 nM of Oligo AS. The protein levels of p53 and/or p21 were increased in a dose-dependent manner. The control mismatched Oligo ASM had minimal effects on the levels of these proteins. Relative levels of each protein were expressed as percentage of control, normalized by corresponding β-actin level.

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Molecular Medicine

ISSN: 1528-3658