Fig. 4

Ultrathin cryosections of HSV-1-infected fibroblasts immunolabeled for gC-1 with HCl and GAR-G5. Convincing gC-1 was not visible in the nuclear membranes (arrows) without BFA treatment (A), although the endoplasmic reticulum and the Golgi complex (empty arrow) were strongly reactive for gC-1. In BFA-treated cells (B, here released for 15 min), the gC-1 accumulated in the nuclear membranes (arrows). Bars: A = 0.3 µm; B = 0.25 µm.