Fig. 5
From: GTK Tyrosine Kinase-induced Alteration of IRS-protein Signalling in Insulin Producing Cells
Insulin-induced activation of AKT and ERK1/2 in RINm5F cells. Serum-starved control (neo-1 and neo-2) RINm5F cells and RINm5F cells overexpressing GTK (GTKwt, GTKY394F, GTKY504F) were stimulated with insulin as described above. The cells were lysed in SDS-β-mercaptoethanol sample buffer and subjected to Western blot analysis for phospho-AKT, AKT, phospho-ERK1/2 and ERK1/2. (A) Densitometric scanning of phosphorylated AKT was performed and the mean normalised against the total amount of AKT was calculated from 4 independent experiments. The stimulation of AKT in response to insulin was calculated as the percent of maximum stimulation. (B) Densitometric scanning of phosphorylated ERK1/2 was performed and the mean normalised against the total amount of ERK1/2 was calculated. The stimulation of ERK1/2 in response to insulin was calculated as the percent of maximum stimulation. Bars are means ± SEM from 5 independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 when compared with corresponding value of RINm5F-control (neo-1 and neo-2).