Figure 2

Semiquantitative RT-PCR analysis for 12 genes in L1236 and GC B cells (CB, centroblasts). cDNA of L1236 and GC B cells was normalized to β-actin by real-time PCR. Equal amounts of cDNA were introduced in RT-PCR analysis using 4 different cDNA dilutions for L1236 (left panel) and GC B cells (right panel). RT-PCR was performed until a faint band was detectable in the lowest dilution of the L1236 cDNA. By comparing the intensity of the bands of L1236 and GC B cells on an agarose gel, the difference of expression for a given gene was estimated. Normal RT-PCR analysis of β-actin is given in the lowest panel and shows the same amount of PCR product for both cell populations. In total, 12 genes found to be upregulated in the SAGE analysis were analyzed. For some cDNAs primerdimers are visible and represent the lower bands. Tag numbers are normalized to 30000 tags.