Figure 3

Semiquantitative RT-PCR analysis for 12 genes in 4 HL cell lines (L428, KMH2, HDLM2, and L1236). The cDNA of the 4 HL cell lines was normalized to β-actin by real-time PCR. RT-PCR analysis of β-actin is given in the lowest panel and shows the same amount of PCR product for all cell populations. A limited cycle number was performed to retain the differences in band intensities in the gel analysis. For each gene, the PCR results are shown for the lowest cycle number at which a PCR product was obtained for all 4 cell lines. These cycle numbers were between 28 and 35 cycles. For some cDNAs primerdimers are visible and represent the lower bands. For 2 transcripts, PTP4A and L-myc, L1236 is the cell line which shows the highest expression of the 4 HL cell lines. Therefore, for these genes it cannot clearly be stated that the expression level in the 3 other HL cell lines is much higher than that in GC B cell because GC B cells were not included in this analysis.