Profiling of Hodgkin’s Lymphoma Cell Line L1236 and Germinal Center B Cells: Identification of Hodgkin’s Lymphoma-specific Genes

Table 1 The 20 transcripts displaying the largest decrease in expression in the cell line L1236 compared with GC B cells


Fold difference	Tag sequence	CB	L1236	Unigene cluster: gene name
79	AAGGGAGCAC	79	0	Hs.181125: lg lambda
58	GGGCATCTCT	58	1	Hs.76807: MHC class ll, DR alpha
39	ATGAGTGCTG	39	0	Hs.86693: EST
39	TTGGTGAAGG	154	4	Hs.288031: sterol-C5-desaturase-like Hs.75968: thymosin, β 4
37	TGTACCCCGC	37	0	Hs.155975: protein tyrosine phosphatase
36	GACCCAACTG	36	1	Hs.89575: CD79B
34	GCGGTTGTGG	34	1	Hs.79356: LAPTm5
33	AAACCCCAAT	33	0	Hs.348136: Homo sapiens mRNA Hs.77735: hypothetical protein FLJ11618
32	GGGGCAACAG	32	0	Hs.276770: CDW52 (CAMPATH-1) Hs.29288: hypothetical protein FLJ21865
27	AAACCAGAGG	27	1	Hs.40202: lymphoid-restricted membrane protein Hs.4291: golgi phosphoprotein 5
26	GCAGTGGGAA	26	0	Hs.890: lymphotoxin β
24	TCTCTCAAAG	24	0	Hs.82212: CD53
23	ACTTTTTCAA	140	6	Hs.136824: ESTs Hs.156814: KIAA0377 gene product
22	TTTCAATAGA	22	0	Hs.288025: Homo sapiens cDNA Hs.50601: hypothetical protein MGC10986
21	GCAGTTCTGA	21	0	Hs.308026: MHC class II, DR β 5 Hs.349123: MHC, class II, DR β 1
20	TATGAGGACA	20	0	Hs.79630: CD79A
19	CTGACTGTCC	19	1	Hs.84298: CD74
18	GGACCAGGCT	18	0	Hs.62771: Homo sapiens mRNA
18	TTAAATCCCA	18	1	Hs.170121: CD45
18	ACGCTCTCGA	18	1	Hs.153053: CD37

n indicates the number of times the tag was identified per 30000 tags. The fold difference was calculated as described in Materials and Methods. Many of the genes down-regulated in L1236, as opposed to GC B cells, demonstrate the lost B lineage-identity of HRS cells, as they represent genes normally expressed B cell-, lymphocyte-, or hematopoietic-specifically and are presented in detail elsewhere (7). CB, centroblasts.

ISSN: 1528-3658