Samplesa | Samples positive/samples analyzed |
|---|
rhoC | ATBF1 | p21SNFT |
|---|
HRS cells | | | |
Case 1 | 3/3 | 3/3 | 3/3 |
Case 2 | 3/3 | 3/3 | 2/3 |
Case 3 | 3/3 | 3/3 | 3/3 |
Case 4 | 3/3 | 3/3 | 2/3 |
Controls (cases 1 to 4) | | | |
KMH2 cells | 6/6 | 5/5 | 5/6 |
Non-HRS cellsb | 3/19 | 1/12 | 0/12 |
GC B cells | 0/6 | 0/6 | 0/6 |
Buffer controls | 0/14 | 0/12 | 1/12 |
- aGroups of 50 HRS cells and 50 non-HRS cells (small cells isolated from the same tissue sections as the HRS cells) were analyzed. Pools of 50 GC B cells isolated from sections of 2 tonsils were analyzed as additional controls. Intact RNA in the tonsillar samples was confirmed by successful amplification of a fragment of the CD52 RNA from several GC B-cell samples (not shown). Buffer controls represent reaction tubes with buffer but without cells. Groups of 10 FACS-sorted KMH2 cells served as positive controls for cDNA synthesis and RT-PCR.
- bThe few positive non-HRS cell samples may be due to cellular contamination of the samples by fragments from HRS cells or expression of the respective genes by some cells among the non-HRS cells.
