Fig. 2

Changes in PAI-1 mRNA after insulin treatment of ob/ob mice. ob/ob mice (24–28 weeks old) were injected intraperitoneally with 10 units of insulin and the indicated tissues were removed 3, 6, and 24 hr later. (A) Total RNA was prepared and analyzed for PAI-1 gene expression by quantitative RT-PCR as described in Materials and Methods. Each time point on the graph represents the mean ±SD of six animals. ◼: adipose tissue; ♦: lung. (B) In situ hybridization was performed on paraffin sections of epididymal fat pads from the untreated ob/ob mice (1 and 2) and the ob/ob mice treated for 6 hr with insulin (3 and 4). Slides were exposed for 3 weeks at 4^°C and stained with hematoxylin and eosin. a, adipocyte; e, endothelial cell; arrowheads indicate positive signal for PAI-1 mRNA, and arrows point to endothelial cells. Original magnification: 400 × for all sections..