Fig. 5
From: Potent Induction of Apoptosis by β-Lapachone in Human Multiple Myeloma Cell Lines and Patient Cells
Apoptosis induced by β-lapachone is accompanied by mitochondrial cytochrome C release and poly (ADP ribose) polymerase (PARP) cleavage. (A) ARH-77 cells were treated with dimethyl sulfoxide (DMSO) (lane 1) or β-lapachone at 4 µM for 0.5 h (lane 2), 2 h (lane 3), or 4 h (lane 4). Mitochondrial cytochrome C release was determined by Western blot assay, as described in Materials and Methods. (B) ARH-77 cells were treated with DMSO (lane 1) or β-lapachone at 2 µM for 2 h (lane 2), 6 h (lane 3), 12 h (lane 4), 24 h (lane 5), or 48 h (lane 6). Immunoblot analyses of the lysates were performed with anti-PARP antibody.