Fig. 2

Immune complexes induced ONOO⁻production by IFN-γ-activated macrophages. To measure ONOO⁻ production of the cellular respiratory burst activity, Rhodamine measurements were monitored as described in Materials and Methods. Interferon- γ-activated J774.A1 cells, (5 × 10⁵ cells in 500 µl) were loaded with dihydroxy-Rhodamine 123, and the suspension was pre-warmed at 37°C, before a 50 µl addition of IgG₁ immune complexes and/or of L-NAME (1 mM) or Free Bo-SOD1 (300 U/ml), rhodamine formation being measured as described in Materials and Methods. The data presented the mean ± SEM of four different experiments.