Fig. 5
Effect of IgG1 IC on (A) superoxide anion, (B) nitric oxide, and (C) peroxynitrite production by interferon-γ(IFN-γ)activated macrophages loaded or not with Bo-SOD1. As described in Materials and Methods, IFN-γ-activated J774.A1 cells were loaded or not for 24 hr with specific Bo-SOD1 IC (20 µg of anti-Bo-SOD1 + 100 µg of Bo-SOD1). Then, cells were washed and stimulated with IgG1 IC to produce (A) superoxide anion as revealed by reduction of ferricytochrome c, (B) nitric oxide as measured by \({\rm{NO}}_2^ - \) production, and (C) peroxynitrites as revealed by rhodamine measurement. Data represent the mean ± SEM of three different experiments.