Fig. 4

Results from immunization of BALB/c mice with pCMV-S and pCMV-S2.S. (A) BALB/c mice were immunized with 100 µg pCMV-S by IM (gray bars) or ID (white bars) injection. Spleens were removed 4 weeks later and CTL activity was determined. Bars represent the mean HBsAg-specific lysis as a percentage of the total possible lysis (% specific lysis) at an effector-to-target ratio (E:T) of 50:1, and black dots represent % specific lysis for individual mice. (B) BALB/c mice were immunized with 100 µg pCMV-S2.S by IM (gray bars) or 1.6 µg by GG (diagonally striped bars) delivery. Mice immunized by GG were boosted in identical manner 2 weeks (light gray striped bars) or 4 weeks (black striped bars) later. CTL activity was determined on spleens removed 6 weeks after priming for IM and ID groups, or at 2 or 4 weeks after boost (which was carried out 4 weeks post-prime) for GG groups. Bars represent the mean HBsAg-specific lysis as a percentage of the total possible lysis (% specific lysis) at an effector-to-target ratio (E:T) of 50:1, and black dots represent % specific lysis for individual mice.