Predominance of Vγ9/Vδ2 T Lymphocytes in the Cerebrospinal Fluid of Children with Tuberculous Meningitis: Reversal after Chemotherapy

Table 3 V γ 9/V δ 2 expansion factor to several different phosphoantigens in vitro

Group	Source	Vγ9/Vδ2 EF after In Vitro Culture with
Group	Source	Rib-1-P	Xyl-1-P	IPP	DMAPP	TUBAg
TBM patients
1	CSF	14	13	24	28	35
	PBMC	7	2	5	6	8
2	CSF	38	30	61	54	68
	PBMC	22	12	30	28	40
3	CSF	15	10	40	30	64
	PBMC	10	8	32	26	44
4	CSF	17	21	44	49	79
	PBMC	12	20	45	53	72
5	CSF	35	27	60	48	90
	PBMC	20	18	34	40	54
6	CSF	15	17	78	80	143
	PBMC	7	12	12	18	32
Controls
7	CSF	1	3	1	7	5
	PBMC	1	1	2	4	5
8	CSF	3	1	2	10	5
	PBMC	1	1	6	4	7
9	CSF	5	2	1	6	8
	PBMC	6	7	ND	ND	5
10	CSF	3	7	7	4	2
	PBMC	1	4	5	5	12
11	CSF	2	3	1	9	11
	PBMC	2	4	4	7	7
12	CSF	5	6	8	8	11
	PBMC	6	4	7	9	9
13	CSF	1	4	5	3	5
	PBMC	3	7	7	9	14

Paired samples of PBMC and CSF cells from children affected by TBM were stimulated in vitro with the indicated phosphoantigens, and the Vγ9/Vδ2 expansion factor (EF) was determined as described in Materials and Methods. ND, not determined.

ISSN: 1528-3658