Fig. 10

Proteolysis of HSPG by cathepsin S in vivo attenuates bFGF-dependent cell-cell attachment mediated by FGFRs and HSPGs. (A) Co-cultures of 32D-FGFR-1 suspension cells with four different cell types—CHO or BAE wild-type (WT) cells, or CHO or BAE cells transfected with cathepsin S (CS)—performed in the presence or absence of 10 ng/ml of bFGF. (B) Comparison of co-cultures of 32D-FGFR-1 suspension cells with either wild-type or cathepsin S-transfected BAE cells without bFGF, with bFGF, with bFGF using BAE cells grown in the presence of E64, or with bFGF using BAE cells preincubated with recombinant cathepsin S (CatS).