Fig. 2
Decreased cathepsin mRNA in macrophage and microglia cell lines after LPS treatments for 24 hr. RAW 264.7 (A) or N13 (B) cells were treated with various doses of LPS for 24 hr as described in Materials and Methods. Total RNA was isolated and 15 µg/lane was subjected to Northern analysis. RNA blots were probed with 32P-labeled cDNAs specific for cathepsin S, cathepsin B, or 18S rRNA. Densitometry of RNA bands was performed by phosphorimager. In the graphs, relative intensity in the absence of LPS treatment is taken as control (100%) and data are normalized to levels of 18S rRNA. Data represent mean ± SEM of three independent experiments.