Inflammatory Mediators Regulate Cathepsin S in Macrophages and Microglia: A Role in Attenuating Heparan Sulfate Interactions

Table 1 Effects of various cytokines on cathepsin S, B, and L mRNA levels

Agent	Concentration	% Relative mRNA in the Absence of Treatment
Agent	Concentration	Cathepsin S	Cathepsin B	Cathepsin L
TNF-α	0.1 ng/ml	70.2	59.5	66.4
TNF-α	1.0 ng/ml	38.1	47.3	36.7
TNF-α	10 ng/ml	59.9	61.0	67.5
IL-1α	0.1 ng/ml	44.0	36.9	40.0
IL-1α	1.0 ng/ml	49.5	55.1	57.1
IL-1α	10 ng/ml	36.8	8.5	18.0
GM-CSF	0.1 ng/ml	100	64.3	82.9
GM-CSF	1.0 ng/ml	69.6	53.2	62.3
GM-CSF	10 ng/ml	46.7	38.2	45.3
IFN-α	3 U/ml	68.2	53.1	59.0
IFN-α	30 U/ml	56.4	49.6	56.3
IFN-γ	30 U/ml	23.7	25.5	24.0

N13 microglial cells were treated for 24 hr in MSFM in the absence or presence of the indicated concentrations of TNF-α, IL-1α, IFN-γ, GM-CSF, and IFN-α. Northern analysis was performed and intensities of specific bands were quantified by densitometry. All values were then standardized to 18S rRNA and data are presented as % relative level of mRNA in the absence of treatment.

ISSN: 1528-3658