Fig. 5
From: Peptidomimetic Fluoromethylketone Rescues Mice from Lethal Endotoxic Shock
Effect of peptidomimetic fluoromethylketones on LPS-induced hepatic apoptosis.
Mice were injected ip with vehicle 1 (A) or LPS (500 µg) in vehicle 1 (B-E), followed by 3 doses of vehicle 2 (B,C), IDN 1965 (10 mg/kg in vehicle 2) (D), or IDN 1529 (10 mg/kg in vehicle 2) (E) at 4-hr intervals beginning 1 hr after LPS. Livers were fixed 11 hr after LPS challenge and subjected to TUNEL. (B) Negative control in which TdT was omitted. TUNIL-positive cells appear brown. (F) Quantitation of TUNIL-positive cells per 400 × field. Lettering in F refers to conditions described in A-E. Asterisks denote statistically significant (p < 0.05) reduction in TUNEL in livers from mice treated with either IDN 1965 or IDN 1529 compared to mice treated with vehicle. Results are pooled from two independent experiments; each bar represents ≥5 mice. Sections were photographed at 400 ×.