Fig. 1

Effect of CF mutations on NBD1 interaction in the yeast two-hybrid system. The yeast strain YRG-2 was cotransformed with the pBDNBD1 and pADNBD1 plasmids as indicated. pADNBD1 and pBDNBD1 were co-expressed with pBDGal4 p53 and pADGal4 T-antigen, respectively, as controls. Equal aliquots of serial dilutions of each yeast strain were plated on -Leu -Trp medium (A) and on -Leu -Trp -His selective medium containing 5 mM AT (B). Triangles indicate increasing dilution of each culture. Plates were incubated for 3 days at 30°C.