Fig. 6

Effect of TS3 on wildtype CFTR mediated cAMP activated chloride current. Polarized monolayers of FRT cells expressing CFTR wt (FRT-WT) were incubated for 48 hours with 5 µM TS3 or the equivalent amount of DMSO (control). In some experiments, the monolayers were subsequently incubated for 9 hours in TS3-free growth medium. Monolayers were mounted in Ussing chambers and transepithelial chloride currents were measured after activation with 10 µM forskolin and 100 µM IBMX. Results represent the mean ± SEM of n = 6 experiments. The asterisk indicates that the TS3 treatment resulted in a significant difference from the respective control (p < 0.05, two-tailed t-test).