Fig. 7
Effect of short-term TS3 treatment of cells on cAMP activated chloride channel function of CFTRΔF508 or wildtype CFTR. Polarized monolayers of FRT-ΔF cells expressing CFTRΔF508 (ΔF508) or FRT-WT cells expressing CFTR wt (WT) were incubated for 6 hours with 5 µM TS3 or the equivalent amount of DMSO (0.03%). Monolayers were mounted in Ussing chambers and transepithelial chloride currents were measured after activation with 10 µM forskolin and 100 µM IBMX. The maximal cAMP-stimulated chloride conductance observed for untreated FRT-WT monolayers was 85 µA/cm2. Results are expressed as percentage of currents for untreated monolayers and represent the mean ± SEM for the indicated number of experiments (n). The asterisk indicates that the TS3 treatment resulted in a significant difference from the respective control (p < 0.05, two-tailed t-test).