Fig. 8

The combined effect of TS3 and genistein to increase CFTRΔF508 cAMP-activated chloride current. Polarized monolayers of FRT cells (FRT) or FRT-ΔF cells expressing CFTRΔF508 (FRT-ΔF508) were incubated for 48 hours with 5 µM TS3 or the equivalent amount of DMSO and subsequently incubated for 9 hours in TS3-free growth medium. Monolayers were mounted in Ussing chambers and transepithelial chloride currents were measured after activation with 10 forskolin and 100 µM IBMX or with 50 µM genistein followed by 10 µM forskolin and 100 µM IBMX, as indicated. Results represent the mean ±SEM for the indicated number of experiments (n). The asterisks indicate that TS3 treatment resulted in a significant increase in chloride current (p < 0.01, two-tailed t-test).