Fig. 1

Generation of monospecific antisera for four human 2-Cys Prxs. (A) Sequence alignment. Deduced amino acid sequences of Prx 1 (GenBank X67951), Prx 2 (Z22548), Prx 3 (D49396) and Prx 4 (U25182) were aligned using MegAlign. Identical residues are shaded. Asterisks mark VCP motifs. Sequences of synthetic peptide immunogens are underlined. (B) Purification of recombinant proteins. SDS/PAGE of Prxs 1–4 in lanes of the same number after Ni²⁺-NTA chromatography and electroelution from gel slices. M, molecular weight markers. (C) Isoform selectivity of antisera. One microgram of purified recombinant human Prx 1, 2, 3, or 4 was loaded in each lane of the same number, subjected to SDS/15% PAGE and immunoblotted with antisera (α) against Prx 1, 2, 3, and 4.