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Fig. 2 | Molecular Medicine

Fig. 2

From: Nonredundant Antioxidant Defense by Multiple Two-Cysteine Peroxiredoxins in Human Prostate Cancer Cells

Fig. 2

Expression of 2-Cys Prx isoforms in human cancer cells. (A) Expression of mRNA. Total RNA from prostate cancer cells Du145, LNCaP, PC3, and TSU-Pr1 and from melanoma cells SK-MEL-28 and −64 was subjected to RT-PCR using primers specific for Prx 1 (lanes 1), 2 (lanes 2), 3 (lanes 3), or 4 (lanes 4). C, control with RT omitted. M, molecular markers. (B) Expression of protein. Extracts from LNCaP (lanes 1), TSU-Pr1 (lanes 2), Du145 (lanes 3), and PC3 (lanes 4) were separated by SDS/15% PAGE and immunoblotted with antisera specific for the Prx isoform designated beneath each panel. Each preimmune serum was nonreactive, as illustrated for Prx 1 (pre). Equal loading was confirmed with anti-tubulin antibody (not shown).

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Molecular Medicine

ISSN: 1528-3658