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Fig. 3 | Molecular Medicine

Fig. 3

From: Noninvasive Dynamic Fluorescence Imaging of Human Melanomas Reveals that Targeted Inhibition of bFGF or FGFR-1 in Melanoma Cells Blocks Tumor Growth by Apoptosis

Fig. 3

Images of VEGF, bFGF, and FGFR-1-stained tissue sections of melanomas injected with Tyr-bFGF or Tyr-FGFR-1 antisense construct. Adjacent tissue sections, prepared from control tumors and tumors that been injected twice weekly for a period of 4 weeks with Tyr-bFGF or Tyr-FGFR-1 antisense construct, were probed with Cy3-conjugated VEGF-D, Cy5-conjugated bFGF, and Cy7-conjugated FGFR-1 antibody. Depicted in panels a, d, and g are tissue sections, obtained from the center of an MGP melanoma, injected with DC-Chol liposomes. Panel a shows staining for VEGF-D (pseudocolored red), d for bFGF (pseudocolored yellow), and g for FGFR-1 (pseudocolored green). Displayed in panels b, e, and h are tissue sections of an MGP melanoma that had been inoculated with the Tyr-bFGF antisense-oriented plasmid. Panel b depicts a tissue section, stained with Cy3-VEGF-D antibody, e a tissue section probed with Cy5-bFGF antibody, and h a tumor section probed with Cy7-FGFR-1 antibody. Images of stained sections, obtained from an MGP melanoma injected with Tyr-FGFR-1 antisense construct, are depicted in panels c, f, and i. The melanoma section shown in panel c was probed with Cy3-VEGF-D antibody, the tissue section, shown in panel f, with Cy5-bFGF antibody, and the melanoma section presented in panel i, with Cy7-FGFR-1 antibody.

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Molecular Medicine

ISSN: 1528-3658