Fig. 4

In vivo and in vitro images of Tyr-bFGF and Tyr-FGFR-1-targeted melanomas demonstrate melanoma cell apoptosis. MGP melanomas, inoculated twice a week for 2 weeks with Tyr-bFGF or Tyr-FGFR-1 antisense construct and Cy7-S100 and Cy5-CD31 antibody and, similarly, control tumors that received only Cy7-S100 and Cy5-CD31 antibody, or injections with DC-Chol liposomes or pREP7 vector and the two fluorochrome-conjugated antibodies, were injected with acridine orange. Two hours later, the tumors were imaged noninvasively in the Cy3 channel. (a) Image of an acridine orange-injected MGP melanoma, injected with DC-Chol liposomes. (b) Image of an acridine orange-inoculated Tyr-bFGF-targeted MGP melanoma.(c) Image of an acridine orange-injected Tyr-FGFR-1-targeted MGP melanoma. Tissue sections, prepared from MGP melanomas that had been injected twice a week for 4 weeks with Tyr-bFGF or Tyr-FGFR-1 antisense construct, were subjected to rhodamine-based TUNEL staining. (d) Image of a tissue section of an MGP melanoma, inoculated with DC-Chol liposomes. (e) A rhodamine-stained image of a tissue section, prepared from a Tyr-bFGF antisense-targeted MGP melanoma. (f) A rhodamine image of a section obtained from a Tyr-FGFR-1 plasmid-injected MGP melanoma. Images of acridine orange-injected tumors were pseudocolored orange, and rhodamine images of melanoma sections were assigned the pseudocolor blue.