Fig. 2

Comparison of in situ ligation and cresyl violet staining for the detection of stroke induced brain damage. (A, B, C, D) In situ ligation based detection of blunt-ended doublestrand DNA breaks. (A′, B′, C′, D′) Cresyl violet staining 6 hr after onset of focal ischemia (A′), 24 hr after onset of focal ischemia (B′), 48 hr after onset of focal ischemia (C′), and 72 hr after onset of focal ischemia (D′). Note the precise delineation of cellular damage on a cell-by-cell basis with the in situ ligation technique, and the lack of said precision using cresyl violet. (Bar, 850 µm).