Fig. 1

Effects of farnesyl protein transferase inhibition on p42/p44 MAP kinase activation. FTIs inhibit phosphorylation of p42/44 MAP kinase after CSF-1 but not after TPA stimulation in human peripheral blood monocytes. Cells were either incubated or not incubated with FTI (20 µM) for 16 hr and stimulated afterward with CSF-1 or TPA for 10 min. Phosphorylation of MAPKs was measured by Western blot in eight volunteers. Antibody binding was quantified using image analysis software. All laboratory based values are given as means ± SEM. (A) Effects of FTI on TPA-induced p42/44 MAP kinase phosphorylation. (B) Effects of FTI on CSF-1-induced p42/44 MAP kinase phosphorylation. (C) A representative example of a Western blot analysis of phosphorylated p42/44 MAP kinase after CSF-1 stimulations (increasing concentrations in ng/ml) in absence and presence of FTI.