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Fig. 1 | Molecular Medicine

Fig. 1

From: Betulinic Acid-induced Mcl-1 Expression in Human Melanoma — Mode of Action and Functional Significance

Fig. 1

Effects of betulinic acid on the expression of Mcl-1 and on various Bcl-2 family members at the protein and RNA level. (A) The melanoma cell line M20 was either untreated (Ctrl), treated with betulinic acid (BetA), with LY294002, or with both substances at concentrations of 10 µg/ml each for 8h. Western blot analysis of the expression of Bax and Bak. 10 µg total protein lysate per lane was loaded. (B) RNase protection analysis of Bcl-2 family members in untreated or betulinic acid treated (10 µg/ml) melanoma cells. Lower panel: concomitantly performed Mcl-1 Western blot analysis (WB) of an aliquot from the same cellular lysate.

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Molecular Medicine

ISSN: 1528-3658