Fig. 2

Analysis of Mcl-1 expression in wortmannin, LY29004 and betulinic acid-treated cells. (A) Dose-response relationship for the inhibition of betulinic acid-induced Mcl-1 expression by LY294002. Betulinic acid (BetA) was added at fixed concentrations (10 µg/ml) 60 min following addition of the indicated concentrations of LY294002 (0, 1, 3 and 10 µg/ml, respectively). (B) Mcl-1 expression after 18 h incubation in the absence and presence of betulinic acid (10 µg/ml) and the inhibitors (I) LY294002 or wortmannin. LY29004 was added at 10 µg/ml final concentration and wortmannin up to a final concentration of 100 nM.