Analysis of Mcl-1 expression and growth in antisense oligonucleotide-treated melanoma cells. (A) Western blot analysis of Mcl-1 expression in antisense-treated cells (AS), in untreated control cells (Ctrl), and in mismatch-treated cells (MM). In order to demonstrate equal loading, the blot was simultaneously hybridized with an antibody against alpha-tubulin. (B) Growth of betulinic acid (BetA), antisense (AS) and mismatch (MM) treated melanoma cells (melanoma cell line 518A2). Data are given as surviving fraction of untreated control cells. Cell numbers were counted after 16 h of treatment. Data are means of three independent experiments. Bars indicate standard deviations (SD). Final concentrations of antisense oligodeoxynucleotides in the experiments were 80 nM and the concentration of betulinic acid was 2,5 µg/ml.