Fig. 5
From: β-Lapachone Induces Cell Cycle Arrest and Apoptosis in Human Colon Cancer Cells
Differential modulation of cell cycle and apoptosis regulatory proteins by β-lapachone in human colon cancer cells. SW480, SW620, and DLD1 cells were treated with DMSO alone (−) or 5 µM β-lapachone (+) for 4 hr, further incubated in drug-free medium for 20 hr, and then analyzed for nuclear (i.e., cyclin A, cyclin B1, cdc2, cdk2, cyclin D1, p21, p27, and actin*), and wholecell (i.e., p53, Bcl-2, Bax, and actin**) protein levels by immunoblotting. Nuclear and whole-cell actin levels served as loading controls for nuclear and whole-cell protein, respectively.