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Fig. 2 | Molecular Medicine

Fig. 2

From: Zinc Finger Transcription Factors as Molecular Targets for Nitric Oxide-mediated Immunosuppression: Inhibition of IL-2 Gene Expression in Murine Lymphocytes

Fig. 2

Subtoxic concentrations of SNOC inhibit IL-1β-dependent IL-2 mRNA expression in lymphocytes. EL4-6.1 cells were incubated for 3 hr without (lane 1) or with 1000 U/ml IL-1β in the absence (lanes 2, 7) or presence of various concentrations of SNOC (lanes 3, 4, 8) or SNOC−NO (lane 5). Subsequently, total cellular RNA was isolated and IL-2- and GAPDH-specific RT-PCR was performed. IL-2 mRNA was quantitated in relation to GAPDH mRNA. Apoptosis-inducing high concentrations of SNOC reduced both IL-2 and GAPDH mRNA expression, whereas subtoxic concentrations of SNOC only decreased IL-2 mRNA in a concentration-dependent manner. Values are mean ± SD of five independent experiments.

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Molecular Medicine

ISSN: 1528-3658