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Fig. 3 | Molecular Medicine

Fig. 3

From: Zinc Finger Transcription Factors as Molecular Targets for Nitric Oxide-mediated Immunosuppression: Inhibition of IL-2 Gene Expression in Murine Lymphocytes

Fig. 3

SNOC-mediated inhibition of IL-2 mRNA expression is reversible. EL4-6.1 cells were incubated without (lane 1) or with 1000 U/ml IL-1β in the absence (lane 2) or presence of 1 mM of SNOC (lane 3) or SNOC−NO (lane 4). After 3 hr, cells were carefully washed and incubated for 24 hr in fresh culture medium. Cells were then incubated again without (lane 1) or with IL-1β (lanes 2–4) for 3 hr. IL-2 mRNA was quantitated in relation to GAPDH mRNA. Under these conditions, inhibition of IL-2 mRNA expression was fully reversible. Values are mean ± SD of three independent experiments.

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Molecular Medicine

ISSN: 1528-3658