Fig. 2

Effect of a functional mutation in CFTR on global gene expression. (a) Analysis of the complete cDNA arrays for cells expressing mutant and wild-type CFTR. The horizontal axis is the ratio of expression of specific genes in wild-type CFTR-transfected cells to parental cells alone. The vertical axis is the ratio of expression of specific genes in ΔF508-CFTR-transfected cells to parental cells alone. Vertical and horizontal dotted lines represent 1 SD around the respective averages, calculated from data in b and c, below. D, genes on the diagonal. The solid box (O) in D marks genes that are not significantly different (i.e., <1 SD) between wild-type and ΔF508-CFTR cells. A, genes above the diagonal in which the expression level in ΔF508-CFTR cells is significantly higher than in wild-type CFTR cells. B, genes below the diagonal in which the expression level in wild-type CFTR cells is significantly higher than in ΔF508-CFTR cells. Genes in the middle square (O) are those whose expressions are the same in all three cell types. (b) Histogram of ratios of gene expression for wild-type CFTR-transfected cells relative to parental cells. (c) Histogram of ratios of gene expression for ΔF508-CFTR cells relative to parental cells. (d) Histogram of ratios of gene expression for ΔF508-CFTR cells relative to wild-type CFTR-transfected cells.