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Fig. 1 | Molecular Medicine

Fig. 1

From: Human Breast Carcinoma Cells Express Type II IL-4 Receptors and Are Sensitive to Antitumor Activity of a Chimeric IL-4-Pseudomonas Exotoxin Fusion Protein in vitro and in vivo

Fig. 1

Cytotoxicity of IL-4(38-37)-PE38KDEL in breast tumors. Ten thousand cells from R-BT primary breast tumor cell culture (A), MCF-7 or MDAMB231 (B) breast cancer cell lines were incubated with various concentrations of IL-4(38-37)-PE38KDEL. Protein synthesis was measured after 20 hr of culture by incorporation of [3H]-leucine (1 μCi for an additional 4 hr), as described in the “Materials and Methods” section. For competition experiments, R-BT cells were preincubated for 45 min with 2 μg/ml of recombinant IL-4 before addition of IL-4(38-37)-PE38KDEL (A). The results are presented as mean ± SD% control of untreated cells from quadruplicate determinations. Mean total counts per minute (cpm) ± SD incorporated in untreated R-BT cells was 11,765 ± 1,138, in MCF-7 cells 53,924 ± 8,835 and in MDA-MB231 cells 113,366 ± 3,261.

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Molecular Medicine

ISSN: 1528-3658