Fig. 2
From: In Vivo Analysis of DNase I Hypersensitive Sites in the Human CFTR Gene
Mapping of the 5′ end of the CFTR YAC. (A) PCR amplification of 37AB12 YAC DNA and human genomic DNA with primers specific for the −79.5 kb region (214 bp product, primers MOU4-Ava and MOU8-Eco), the −20.5 kb region (274 bp product, primers HUG20C and HUG20D), and the 185 + 10 kb region (199 bp product, primers TSR7-PacI and TSR8-SacII). Each PCR reaction also contains primers for exon 4 (438 bp product, primers 4i-5 and 4i-3). For each set of three reactions, lane C contains the product of the no-DNA control, lane G contains the PCR products obtained using human genomic template DNA, and lane Y contains those using 37AB12 YAC template DNA. (B, C) Southern blots of 37AB12 DNA cleaved with HindIII and probed with pB14E4.9 (B) and pB14E5.3 (C). In both panels lane 1 contains 37AB12 YAC DNA; lane 2, a control YAC from outside the CFTR locus also cleaved with HindIII; and lane 3, probe DNA. In (B) lane 4 is a 1 kb ladder (Gibco BRL). The multiple bands in the two YAC lanes are probably due to cross-hybridization to repetitive elements in the human DNA.