Fig. 4
From: In Vivo Analysis of DNase I Hypersensitive Sites in the Human CFTR Gene
In vivo evaluation of the −20.5 Kb DHS. (A–I) Chromatin extracted from transgenic mouse tissues, digested with DNase I, cleaved with EcoRI, separated on 1% agarose gels. Southern blotted, and probed with cW44 H1.1 to detect the DHS at −20.5 kb. For each tissue, lane 1 shows DNA prepared from nuclei that had not been treated with DNase I and lanes 2–5 show DNA treated with increasing amounts of DNase I: lane 2, 15 units; lane 3, 30 units; lane 4, 60 units; lane 5, 120 units. Molecular weights were estimated with respect to a 1 kb DNA ladder (Gibco BRL).